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페이지 정보작성자 최고관리자 작성일15-06-29 18:20 조회1,503회 댓글0건
Rewiring of PDZ Domain-Ligand Interaction Network Contributed to Eukaryotic Evolution
Jinho Kim1, Inhae Kim1, Jae-Seong Yang2, Young-Eun Shin1, Jihye Hwang3, Solip Park2, Yoon Sup Choi4, Sanguk Kim1,2,3*
1 Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang, Korea, 2 School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Korea, 3 Division of ITCE, Pohang University of Science and Technology, Pohang, Korea, 4 Cancer Research Institute, Seoul National University, Seoul, Korea
PDZ domain-mediated interactions have greatly expanded during metazoan evolution, becoming important for controlling signal flow via the assembly of multiple signaling components. The evolutionary history of PDZ domain-mediated interactions has never been explored at the molecular level. It is of great interest to understand how PDZ domain-ligand interactions emerged and how they become rewired during evolution. Here, we constructed the first human PDZ domain-ligand interaction network (PDZNet) together with binding motif sequences and interaction strengths of ligands. PDZNet includes 1,213 interactions between 97 human PDZ proteins and 591 ligands that connect most PDZ protein-mediated interactions (98%) in a large single network via shared ligands. We examined the rewiring of PDZ domain-ligand interactions throughout eukaryotic evolution by tracing changes in the C-terminal binding motif sequences of the PDZ ligands. We found that interaction rewiring by sequence mutation frequently occurred throughout evolution, largely contributing to the growth of PDZNet. The rewiring of PDZ domain-ligand interactions provided an effective means of functional innovations in nervous system development. Our findings provide empirical evidence for a network evolution model that highlights the rewiring of interactions as a mechanism for the development of new protein functions. PDZNet will be a valuable resource to further characterize the organization of the PDZ domain-mediated signaling proteome.
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