페이지 정보작성자 이나현 작성일21-09-23 13:20 조회52회 댓글0건
White blood cells act as the first line of defense of the immune system and are often referred to as the army that protects our body. Neutrophils make up the majority (55-70%) of human white blood cells and fight bacteria and fungi. A deficiency of neutrophils causes an abnormality in the immune system. They normally circulate in the blood and travel to the site of infection or inflammation of the tissue. Recently, POSTECH’s research team has developed a method for decoding living neutrophils.
Antibodies help detect specific cells, but antibodies with low cell permeability have limitations in identifying intracellular biomarkers. Identifying biomarkers requires processes of cell fixation and penetration, which change from the live state through the pretreatment process. These differences limit the choice of antibodies when studying living cells.
In response, the research team sought to use small molecule fluorescent compounds with relatively high cell permeability to overcome the shortcomings of antibodies. NeutropG, developed in this study, is selectively labeled on neutrophils through lipid droplet biosynthesis * 2 and has a high rate of enzyme gene expression for long-chain acyl-CoA synthetase (ACSL) * 3 and diglyceride acyltransferase (DGAT). It arises from different things.
We used NeutropG to observe the phagocytotic process of neutrophils. This proved that the stain was stable for a long period of time and did not significantly affect the original function of neutrophils.
This study confirmed that NeutropG selectively stains healthy neutrophils, and through its application, researchers have been able to accurately quantify neutrophil levels in fresh blood samples. .. This high selectivity of neutrophils has shown potential for clinical diagnosis.
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