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I-Bio 겸임 장영태 교수, POSTECH: Selective Staining of Neutrophils in White B…

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작성자 이나현 작성일21-09-23 13:20 조회52회 댓글0건

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White blood cells act as the first line of defense of the immune system and are often referred to as the army that protects our body. Neutrophils make up the majority (55-70%) of human white blood cells and fight bacteria and fungi. A deficiency of neutrophils causes an abnormality in the immune system. They normally circulate in the blood and travel to the site of infection or inflammation of the tissue. Recently, POSTECH’s research team has developed a method for decoding living neutrophils.

 

Professor Chang Young-Tae of the Department of Chemistry, POSTECH (Deputy Director, Center for Self-Organizing Complexity, Institute of Basic Sciences), Sun Hyeok Lee of the Faculty of Convergence Science and Technology, and Min Gao Basic Science of the Institute are collaborating with Ursan University Hospital. , Reported the first fluorescent probe NeutropG for specific identification and imaging of active neutrophils. * 1 Recognizing its academic excellence, this study was published internationally on August 19, 2021. An edition of the world-famous chemistry journal Angewandte Chemie.

Fluorescent probes are reporters that indicate whether a particular ion or substance is recognized by emitting an optical signal. Identifying living neutrophils in humans is important not only for clinical diagnosis, but also for finding treatments for infections and inflammation. However, no small molecule-based probe has been developed to distinguish live neutrophils between granulocytes.

Antibodies help detect specific cells, but antibodies with low cell permeability have limitations in identifying intracellular biomarkers. Identifying biomarkers requires processes of cell fixation and penetration, which change from the live state through the pretreatment process. These differences limit the choice of antibodies when studying living cells. 

 

In response, the research team sought to use small molecule fluorescent compounds with relatively high cell permeability to overcome the shortcomings of antibodies. NeutropG, developed in this study, is selectively labeled on neutrophils through lipid droplet biosynthesis * 2 and has a high rate of enzyme gene expression for long-chain acyl-CoA synthetase (ACSL) * 3 and diglyceride acyltransferase (DGAT). It arises from different things. 

NeutropG uses a unique mechanism called metabolism-oriented living cell identification (MOLD), which is completely different from conventional holdings, and is converted to triacylglycerol (TAG), which is a component of lipid droplets, by the action of these enzymes. Was confirmed. Oriented Live-cell Distinction (HOLD) or Gating Oriented Live-cell Distinction (GOLD). 

We used NeutropG to observe the phagocytotic process of neutrophils. This proved that the stain was stable for a long period of time and did not significantly affect the original function of neutrophils. 

 

This study confirmed that NeutropG selectively stains healthy neutrophils, and through its application, researchers have been able to accurately quantify neutrophil levels in fresh blood samples. .. This high selectivity of neutrophils has shown potential for clinical diagnosis.

“NeutropG is the first case of specific identification and imaging of active neutrophils in blood samples,” explained Professor Chang, who led the study. “In particular, the distinction between metabolism-oriented living cells has the power to selectively identify healthy neutrophils.”

출처: India News Republic(2021.09.14)

 

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